文章基本信息

标题：Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2
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作者：Kathrin Kastning ; Viktoria Kukhtina ; Josef T. Kittler 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2007
卷号：104
期号：8
页码：2991-2996
DOI：10.1073/pnas.0611170104
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：{alpha} -Amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptors undergo constitutive and ligand-induced internalization that requires dynamin and the clathrin adaptor complex AP-2. We report here that an atypical basic motif within the cytoplasmic tails of AMPA-type glutamate receptors directly associates with {micro}2-adaptin by a mechanism similar to the recognition of the presynaptic vesicle protein synaptotagmin 1 by AP-2. A synaptotagmin 1-derived AP-2 binding peptide competes the interaction of the AMPA receptor subunit GluR2 with AP-2{micro} and increases the number of surface active glutamate receptors in living neurons. Moreover, fusion of the GluR2-derived tail peptide with a synaptotagmin 1 truncation mutant restores clathrin/AP-2-dependent internalization of the chimeric reporter protein. These data suggest that common mechanisms regulate AP-2-dependent internalization of pre- and postsynaptic membrane proteins.
关键词：endocytosis ; postsynaptic ; sorting signal ; synaptic plasticity