文章基本信息

标题：Nitric oxide S-nitrosylates serine racemase, mediating feedback inhibition of d-serine formation
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作者：Asif K. Mustafa ; Manish Kumar ; Balakrishnan Selvakumar 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2007
卷号：104
期号：8
页码：2950-2955
DOI：10.1073/pnas.0611620104
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Serine racemase (SR) generates D-serine, a coagonist with glutamate at NMDA receptors. We show that SR is physiologically S-nitrosylated leading to marked inhibition of enzyme activity. Inhibition involves interactions with the cofactor ATP reflecting juxtaposition of the ATP-binding site and cysteine-113 (C113), the site for physiological S-nitrosylation. NMDA receptor physiologically enhances SR S-nitrosylation by activating neuronal nitric-oxide synthase (nNOS). These findings support a model whereby postsynaptic stimulation of nitric-oxide (NO) formation feeds back to presynaptic cells to S-nitrosylate SR and decrease D-serine availability to postsynaptic NMDA receptors.
关键词：neuronal nitric-oxide synthase ; NMDA receptor ; S -nitrosylation