文章基本信息

标题：Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog
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作者：Olivier Serralbo ; José Manuel Pérez-Pérez ; Renze Heidstra 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2006
卷号：103
期号：35
页码：13250-13255
DOI：10.1073/pnas.0602410103
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The Arabidopsis HOBBIT (HBT) gene encodes a homolog of the CDC27 anaphase-promoting complex/cyclosome subunit and is essential for postembryonic development. We induced loss-of-function clones by Cre/lox-mediated recombination of a single complementing HBT transgene in a background homozygous for the strong mutant allele hbt2311. Defects in cell division and cell expansion are the primary consequences of ubiquitous postembryonic HBT excision. In roots, both cell division and cell expansion are rapidly affected. In contrast, in leaf primordia, cell division and cell expansion halt after a lag phase, which results in different severities of defects in the proximodistal and mediolateral axes. Surprisingly, small clones reveal non-cell-autonomous rescue of hbt mutant cells, indicating a previously unrecognized compensation mechanism for reduced activity of an anaphase-promoting complex/cyclosome component critical for cell cycle progression.
关键词：cell cycle ; clonal analysis ; growth control ; root development