文章基本信息

标题：Molecular dynamics analyses of cross-β-spine steric zipper models: β-Sheet twisting and aggregation
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作者：Luciana Esposito ; Carlo Pedone ; Luigi Vitagliano 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2006
卷号：103
期号：31
页码：11533-11538
DOI：10.1073/pnas.0602345103
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The structural characterization of amyloid fibers is one of the most investigated areas in structural biology. The structural motif, denoted as steric zipper, recently discovered for the peptide GNNQQNY [Nelson, R., Sawaya, M. R., Balbirnie, M., Madsen, A. O., Riekel, C., Grothe, R. & Eisenberg, D. (2005) Nature 435, 773-778], is expected to exert strong influence in this field. To obtain further insights into the features of this unique structural motif, we report several molecular dynamics simulations of various GNNQQNY aggregates. Our analyses show that even pairs of [beta]-sheets composed of a limited number of [beta]-strands are stable in the 20-ns time interval considered, which suggests that steric zipper interactions at a [beta]-sheet-[beta]-sheet interface strongly contribute to the stability of these aggregates. Moreover, although the basic features of side chain-side chain interactions are preserved in the simulation, the backbone structure undergoes significant variations. Upon equilibration, a significant twist of the [beta]-strands that compose the [beta]-sheets is observed. These results demonstrate that the occurrence of steric zipper interactions is compatible with flat and twisted [beta]-sheets. Molecular dynamics simulations carried out on two pairs of [beta]-sheets, separated in the crystal state by a hydrated interface, lead to interesting results. The two pairs of sheets, while twisting, associate through stable peptide-peptide interactions. These findings provide insight into the mechanism that leads to the formation of higher aggregates. On these bases, it is possible to reconcile the crystallographic and the EM data on the size of the basic GNNQQNY fiber unit.
关键词：amyloid fiber ; prion ; neurodegenerative diseases