文章基本信息

标题：Global gene expression profiles reveal significant nuclear reprogramming by the blastocyst stage after cloning
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作者：Sadie L. Smith ; Robin E. Everts ; X. Cindy Tian 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2005
卷号：102
期号：49
页码：17582-17587
DOI：10.1073/pnas.0508952102
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Nuclear transfer (NT) has potential applications in agriculture and biomedicine, but the technology is hindered by low efficiency. Global gene expression analysis of clones is important for the comprehensive study of nuclear reprogramming. Here, we compared global gene expression profiles of individual bovine NT blastocysts with their somatic donor cells and fertilized control embryos using cDNA microarray technology. The NT embryos' gene expression profiles were drastically different from those of their donor cells and closely resembled those of the naturally fertilized embryos. Our findings demonstrate that the NT embryos have undergone significant nuclear reprogramming by the blastocyst stage; however, problems may occur during redifferentiation for tissue genesis and organogenesis, and small reprogramming errors may be magnified downstream in development.
关键词：bovine ; embryo ; microarray ; nuclear transfer