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标题：AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate
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作者：Shinobu Hirai ; Kohji Hotta ; Yoshihiro Kubo 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：15
页码：3939-3944
DOI：10.1073/pnas.1612943114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：AMPA-type glutamate receptors (GluAs) mediate fast excitatory transmission in the vertebrate central nervous system (CNS), and their function has been extensively studied in the mature mammalian brain. However, GluA expression begins very early in developing embryos, suggesting that they may also have unidentified developmental roles. Here, we identify developmental roles for GluAs in the ascidian Ciona intestinalis . Mammals express Ca²⁺-permeable GluAs (Ca-P GluAs) and Ca²⁺-impermeable GluAs (Ca-I GluAs) by combining subunits derived from four genes. In contrast, ascidians have a single gluA gene. Taking advantage of the simple genomic GluA organization in ascidians, we knocked down (KD) GluAs in Ciona and observed severe impairments in formation of the ocellus, a photoreceptive organ used during the swimming stage, and in resorption of the tail and body axis rotation during metamorphosis to the adult stage. These defects could be rescued by injection of KD-resistant GluAs. GluA KD phenotypes could also be reproduced by expressing a GluA mutant that dominantly inhibits glutamate-evoked currents. These results suggest that, in addition to their role in synaptic communication in mature animals, GluAs also have critical developmental functions.
关键词：AMPA-type glutamate receptor ; morphogeneis ; ascidian ; development