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标题：Neisseria meningitidis GNA2132, a heparin-binding protein that induces protective immunity in humans
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作者：Davide Serruto ; Tiziana Spadafina ; Laura Ciucchi 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：8
页码：3770-3775
DOI：10.1073/pnas.0915162107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：GNA2132 is a Neisseria meningitidis antigen of unknown function, discovered by reverse vaccinology, which has been shown to induce bactericidal antibodies in animal models. Here we show that this antigen induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease. The protein binds heparin in vitro through an Arg-rich region and this property correlates with increased survival of the unencapsulated bacterium in human serum. Furthermore, two proteases, the meningococcal NalP and human lactoferrin, cleave the protein upstream and downstream from the Arg-rich region, respectively. We conclude that GNA2132 is an important protective antigen of N. meningitidis and we propose to rename it, Neisserial Heparin Binding Antigen (NHBA).
关键词：serum resistance ; vaccine ; Arg-rich region ; NalP ; Lactoferrin