文章基本信息

标题：Unexpected role of clathrin adaptor AP-1 in MHC-dependent positive selection of T cells
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作者：Diana A. Alvarez Arias ; Nami McCarty ; Linrong Lu 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：6
页码：2556-2561
DOI：10.1073/pnas.0913671107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Trafficking of transmembrane receptors to a specific intracellular compartment is conducted by adaptor molecules that bind to target motifs within the cytoplasmic domains of cargo proteins. We generated mice containing a lymphoid-specific deficiency of AP-1 using RNAi knockdown technology. Inhibition of AP-1 expression in thymocytes blocks progression from double-positive immature thymocytes, resulting in complete absence of CD4+ single-positive thymocytes and severe reduction of CD3+CD8+ single-positive thymocytes. Analysis of the contribution of AP-1 deficiency on the interaction between mature CD4+ T cells and antigen-presenting cells revealed that AP-1 is essential to efficient immune synapse formation and associated T cell activation, suggesting a possible mechanism of AP-1 function in thymocyte development.
关键词：adaptor protein ; immunologic synapse ; T cell development ; T cell receptor