文章基本信息

标题：Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors
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作者：Nobuo Misawa ; Hidefumi Mitsuno ; Ryohei Kanzaki 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：35
页码：15340-15344
DOI：10.1073/pnas.1004334107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：This paper describes a highly sensitive and selective chemical sensor using living cells (Xenopus laevis oocytes) within a portable fluidic device. We constructed an odorant sensor whose sensitivity is a few parts per billion in solution and can simultaneously distinguish different types of chemicals that have only a slight difference in double bond isomerism or functional group such as [boxh]OH, [boxh]CHO and [boxh]C([boxH]O)[boxh]. We developed a semiautomatic method to install cells to the fluidic device and achieved stable and reproducible odorant sensing. In addition, we found that the sensor worked for multiple-target chemicals and can be integrated with a robotic system without any noise reduction systems. Our developed sensor is compact and easy to replace in the system. We believe that the sensor can potentially be incorporated into a portable system for monitoring environmental and physical conditions.
关键词：fluidic channel ; odorant receptor ; robot ; Xenopus oocyte