文章基本信息

标题：Severe anemia in the Nan mutant mouse caused by sequence-selective disruption of erythroid Krüppel-like factor
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作者：Miroslawa Siatecka ; Kenneth E. Sahr ; Sabra G. Andersen 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：34
页码：15151-15156
DOI：10.1073/pnas.1004996107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Studies of mouse models of anemia have long provided fundamental insights into red blood cell formation and function. Here we show that the semidominant mouse mutation Nan ("neonatal anemia") carries a single amino acid change (E339D) within the second zinc finger of the erythroid Kruppel-like factor (EKLF), a critical erythroid regulatory transcription factor. The mutation alters the DNA-binding specificity of EKLF so that it no longer binds promoters of a subset of its DNA targets. Remarkably, even when mutant Nan and wild-type EKLF alleles are expressed at equivalent levels, the mutant form selectively interferes with expression of EKLF target genes whose promoter elements it no longer binds. This interference yields a distorted genetic output and selective protein deficiencies that differ from those seen in EKLF-heterozygous and EKLF-null red blood cells and presents a unique and unexpected mechanism of inherited disease.
关键词：mouse model ; neonatal anemia ; red blood cells ; zinc finger mutation