文章基本信息

标题：Conversion of a yeast prion protein to an infectious form in bacteria
本地全文：下载
作者：Sean J. Garrity ; Viknesh Sivanathan ; Jijun Dong 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：23
页码：10596-10601
DOI：10.1073/pnas.0913280107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Prions are infectious, self-propagating protein aggregates that have been identified in evolutionarily divergent members of the eukaryotic domain of life. Nevertheless, it is not yet known whether prokaryotes can support the formation of prion aggregates. Here we demonstrate that the yeast prion protein Sup35 can access an infectious conformation in Escherichia coli cells and that formation of this material is greatly stimulated by the presence of a transplanted [PSI+] inducibility factor, a distinct prion that is required for Sup35 to undergo spontaneous conversion to the prion form in yeast. Our results establish that the bacterial cytoplasm can support the formation of infectious prion aggregates, providing a heterologous system in which to study prion biology.
关键词：Sup35 ; [ PSI ⁺] inducibility factor ; amyloid