文章基本信息

标题：Catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteria
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作者：Bo Li ; Daniel Sher ; Libusha Kelly 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：23
页码：10430-10435
DOI：10.1073/pnas.0913677107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Our understanding of secondary metabolite production in bacteria has been shaped primarily by studies of attached varieties such as symbionts, pathogens, and soil bacteria. Here we show that a strain of the single-celled, planktonic marine cyanobacterium Prochlorococcus--which conducts a sizable fraction of photosynthesis in the oceans--produces many cyclic, lanthionine-containing peptides (lantipeptides). Remarkably, in Prochlorococcus MIT9313 a single promiscuous enzyme transforms up to 29 different linear ribosomally synthesized peptides into a library of polycyclic, conformationally constrained products with highly diverse ring topologies. Genes encoding this system are found in variable abundances across the oceans--with a hot spot in a Galapagos hypersaline lagoon--suggesting they play a habitat- and/or community-specific role. The extraordinarily efficient pathway for generating structural diversity enables these cyanobacteria to produce as many secondary metabolites as model antibiotic-producing bacteria, but with much smaller genomes.
关键词：lantibiotic ; Synechococcus ; combinatorial biosynthesis ; Global Ocean Survey metagenome