文章基本信息

标题：Genetic suppression of the circadian Clock mutation by the melatonin biosynthesis pathway
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作者：Kazuhiro Shimomura ; Phillip L. Lowrey ; Martha Hotz Vitaterna 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：18
页码：8399-8403
DOI：10.1073/pnas.1004368107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Most laboratory mouse strains including C57BL/6J do not produce detectable levels of pineal melatonin owing to deficits in enzymatic activity of arylalkylamine N-acetyltransferase (AANAT) and N-acetylserotonin O-methyl transferase (ASMT), two enzymes necessary for melatonin biosynthesis. Here we report that alleles segregating at these two loci in C3H/HeJ mice, an inbred strain producing melatonin, suppress the circadian period-lengthening effect of the Clock mutation. Through a functional mapping approach, we localize mouse Asmt to chromosome X and show that it, and the Aanat locus on chromosome 11, are significantly associated with pineal melatonin levels. Treatment of suprachiasmatic nucleus (SCN) explant cultures from Period2Luciferase (Per2Luc) Clock/+ reporter mice with melatonin, or the melatonin agonist, ramelteon, phenocopies the genetic suppression of the Clock mutant phenotype observed in living animals. These results demonstrate that melatonin suppresses the Clock/+ mutant phenotype and interacts with Clock to affect the mammalian circadian system.
关键词：arylalkylamine N -acetyltransferase ; N -acetylserotonin O -methyl transferase ; Clock gene ; suprachiasmatic nucleus