文章基本信息

标题：Experimental snapshots of a protein-DNA binding landscape
本地全文：下载
作者：Ignacio E. Sánchez ; Diego U. Ferreiro ; Mariano Dellarole 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：17
页码：7751-7756
DOI：10.1073/pnas.0911734107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Protein recognition of DNA sites is a primary event for gene function. Its ultimate mechanistic understanding requires an integrated structural, dynamic, kinetic, and thermodynamic dissection that is currently limited considering the hundreds of structures of protein-DNA complexes available. We describe a protein-DNA-binding pathway in which an initial, diffuse, transition state ensemble with some nonnative contacts is followed by formation of extensive nonnative interactions that drive the system into a kinetic trap. Finally, nonnative contacts are slowly rearranged into native-like interactions with the DNA backbone. Dissimilar protein-DNA interfaces that populate along the DNA-binding route are explained by a temporary degeneracy of protein-DNA interactions, centered on "dual-role" residues. The nonnative species slow down the reaction allowing for extended functionality.
关键词：energy landscape ; human papillomavirus E2 protein ; kinetic trap ; kinetics ; protein-DNA interaction