文章基本信息

标题：High-resolution x-ray diffraction microscopy of specifically labeled yeast cells
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作者：Johanna Nelson ; Xiaojing Huang ; Jan Steinbrener 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：16
页码：7235-7239
DOI：10.1073/pnas.0910874107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：X-ray diffraction microscopy complements other x-ray microscopy methods by being free of lens-imposed radiation dose and resolution limits, and it allows for high-resolution imaging of biological specimens too thick to be viewed by electron microscopy. We report here the highest resolution (11-13 nm) x-ray diffraction micrograph of biological specimens, and a demonstration of molecular-specific gold labeling at different depths within cells via through-focus propagation of the reconstructed wavefield. The lectin concanavalin A conjugated to colloidal gold particles was used to label the {alpha}-mannan sugar in the cell wall of the yeast Saccharomyces cerevisiae. Cells were plunge-frozen in liquid ethane and freeze-dried, after which they were imaged whole using x-ray diffraction microscopy at 750 eV photon energy.
关键词：coherent imaging ; immunogold labeling