文章基本信息

标题：Identification of the Tau phosphorylation pattern that drives its aggregation
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作者：Clément Despres ; Cillian Byrne ; Haoling Qi 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：34
页码：9080-9085
DOI：10.1073/pnas.1708448114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Determining the functional relationship between Tau phosphorylation and aggregation has proven a challenge owing to the multiple potential phosphorylation sites and their clustering in the Tau sequence. We use here in vitro kinase assays combined with NMR spectroscopy as an analytical tool to generate well-characterized phosphorylated Tau samples and show that the combined phosphorylation at the Ser202/Thr205/Ser208 sites, together with absence of phosphorylation at the Ser262 site, yields a Tau sample that readily forms fibers, as observed by thioflavin T fluorescence and electron microscopy. On the basis of conformational analysis of synthetic phosphorylated peptides, we show that aggregation of the samples correlates with destabilization of the turn-like structure defined by phosphorylation of Ser202/Thr205.
关键词：Tau ; phosphorylation ; aggregation ; Alzheimer’s disease ; NMR