文章基本信息

标题：Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements
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作者：Gustavo Fuertes ; Niccolò Banterle ; Kiersten M. Ruff 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：31
页码：E6342-E6351
DOI：10.1073/pnas.1704692114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Unfolded states of proteins and native states of intrinsically disordered proteins (IDPs) populate heterogeneous conformational ensembles in solution. The average sizes of these heterogeneous systems, quantified by the radius of gyration ( R _G), can be measured by small-angle X-ray scattering (SAXS). Another parameter, the mean dye-to-dye distance ( R _E) for proteins with fluorescently labeled termini, can be estimated using single-molecule Förster resonance energy transfer (smFRET). A number of studies have reported inconsistencies in inferences drawn from the two sets of measurements for the dimensions of unfolded proteins and IDPs in the absence of chemical denaturants. These differences are typically attributed to the influence of fluorescent labels used in smFRET and to the impact of high concentrations and averaging features of SAXS. By measuring the dimensions of a collection of labeled and unlabeled polypeptides using smFRET and SAXS, we directly assessed the contributions of dyes to the experimental values R _G and R _E. For chemically denatured proteins we obtain mutual consistency in our inferences based on R _G and R _E, whereas for IDPs under native conditions, we find substantial deviations. Using computations, we show that discrepant inferences are neither due to methodological shortcomings of specific measurements nor due to artifacts of dyes. Instead, our analysis suggests that chemical heterogeneity in heteropolymeric systems leads to a decoupling between R _E and R _G that is amplified in the absence of denaturants. Therefore, joint assessments of R _G and R _E combined with measurements of polymer shapes should provide a consistent and complete picture of the underlying ensembles.
关键词：single-molecule FRET ; intrinsically disordered proteins ; denatured-state ensemble ; protein folding ; polymer theory