文章基本信息

标题：Molecular mechanism of environmental d-xylose perception by a XylFII-LytS complex in bacteria
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作者：Jianxu Li ; Chengyuan Wang ; Gaohua Yang 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：31
页码：8235-8240
DOI：10.1073/pnas.1620183114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：d -xylose, the main building block of plant biomass, is a pentose sugar that can be used by bacteria as a carbon source for bio-based fuel and chemical production through fermentation. In bacteria, the first step for d -xylose metabolism is signal perception at the membrane. We previously identified a three-component system in Firmicutes bacteria comprising a membrane-associated sensor protein (XylFII), a transmembrane histidine kinase (LytS) for periplasmic d -xylose sensing, and a cytoplasmic response regulator (YesN) that activates the transcription of the target ABC transporter xylFGH genes to promote the uptake of d -xylose. The molecular mechanism underlying signal perception and integration of these processes remains elusive, however. Here we purified the N-terminal periplasmic domain of LytS (LytSN) in a complex with XylFII and determined the conformational structures of the complex in its d -xylose–free and d -xylose–bound forms. LytSN contains a four-helix bundle, and XylFII contains two Rossmann fold-like globular domains with a xylose-binding cleft between them. In the absence of d -xylose, LytSN and XylFII formed a heterodimer. Specific binding of d -xylose to the cleft of XylFII induced a large conformational change that closed the cleft and brought the globular domains closer together. This conformational change led to the formation of an active XylFII-LytSN heterotetramer. Mutations at the d -xylose binding site and the heterotetramer interface diminished heterotetramer formation and impaired the d -xylose–sensing function of XylFII-LytS. Based on these data, we propose a working model of XylFII-LytS that provides a molecular basis for d -xylose utilization and metabolic modification in bacteria.
关键词：two-component system ; molecular mechanism ; d -xylose uptake ; histidine kinase ; cross-membrane signaling