文章基本信息

标题：Diazotrophic Bacterial Community of Degraded Pastures
本地全文：下载
作者：João Tiago Correia Oliveira ; Everthon Fernandes Figueredo ; Williane Patrícia da Silva Diniz 等
期刊名称：Applied and Environmental Soil Science
印刷版ISSN：1687-7667
电子版ISSN：1687-7675
出版年度：2017
卷号：2017
DOI：10.1155/2017/2561428
出版社：Hindawi Publishing Corporation
摘要：Pasture degradation can cause changes in diazotrophic bacterial communities. Thus, this study aimed to evaluate the culturable and total diazotrophic bacterial community, associated with regions of the rhizosphere and roots of Brachiaria decumbens Stapf. pastures in different stages of degradation. Samples of roots and rhizospheric soil were collected from slightly, partially, and highly degraded pastures. McCrady’s table was used to obtain the Most Probable Number (MPN) of bacteria per gram of sample, in order to determine population density and calculate the Shannon-Weaver diversity index. The diversity of total diazotrophic bacterial community was determined by the technique of Denaturing Gradient Gel Electrophoresis (DGGE) of the nifH gene, while the diversity of the culturable diazotrophic bacteria was determined by the Polymerase Chain Reaction (BOX-PCR) technique. The increase in the degradation stage of the B. decumbens Stapf. pasture did not reduce the population density of the cultivated diazotrophic bacterial community, suggesting that the degradation at any degree of severity was highly harmful to the bacteria. The structure of the total diazotrophic bacterial community associated with B. decumbens Stapf. was altered by the pasture degradation stage, suggesting a high adaptive capacity of the bacteria to altered environments.