文章基本信息

标题：Theory of long binding events in single-molecule–controlled rotation experiments on F1-ATPase
本地全文：下载
作者：Sándor Volkán-Kacsó ; Rudolph A. Marcus
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：28
页码：7272-7277
DOI：10.1073/pnas.1705960114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The theory of elastic group transfer for the binding and release rate constants for nucleotides in F₁-ATPase as a function of the rotor angle is further extended in several respects. ( i ) A method is described for predicting the experimentally observed lifetime distribution of long binding events in the controlled rotation experiments by taking into account the hydrolysis and synthesis reactions occurring during these events. ( ii ) A method is also given for treating the long binding events in the experiments and obtaining the rate constants for the hydrolysis and synthesis reactions occurring during these events. ( iii ) The theory in the previous paper is given in a symmetric form, an extension that simplifies the application of the theory to experiments. It also includes a theory-based correction of the reported “on” and “off” rates by calculating the missed events. A near symmetry of the data about the angle of −40° and a “turnover” in the binding rate data vs. rotor angle for angles greater than ∼ 40° is also discussed.
关键词：F-ATPase ; single-molecule imaging ; nucleotide binding ; hydrolysis ; group transfer theory