文章基本信息

标题：Structural characterization of the Rabphilin-3A–SNAP25 interaction
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作者：Cristina Ferrer-Orta ; María Dolores Pérez-Sánchez ; Teresa Coronado-Parra 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：27
页码：E5343-E5351
DOI：10.1073/pnas.1702542114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Membrane fusion is essential in a myriad of eukaryotic cell biological processes, including the synaptic transmission. Rabphilin-3A is a membrane trafficking protein involved in the calcium-dependent regulation of secretory vesicle exocytosis in neurons and neuroendocrine cells, but the underlying mechanism remains poorly understood. Here, we report the crystal structures and biochemical analyses of Rabphilin-3A C2B–SNAP25 and C2B–phosphatidylinositol 4,5-bisphosphate (PIP₂) complexes, revealing how Rabphilin-3A C2 domains operate in cooperation with PIP₂/Ca²⁺ and SNAP25 to bind the plasma membrane, adopting a conformation compatible to interact with the complete SNARE complex. Comparisons with the synaptotagmin1–SNARE show that both proteins contact the same SNAP25 surface, but Rabphilin-3A uses a unique structural element. Data obtained here suggest a model to explain the Ca²⁺-dependent fusion process by membrane bending with a myriad of variations depending on the properties of the C2 domain-bearing protein, shedding light to understand the fine-tuning control of the different vesicle fusion events.
关键词：C2 domains ; membrane fusion ; Rabphilin-3A ; SNAP-25 ; X-ray crystallography