文章基本信息

标题：Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili
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作者：Vivianne J. Goosens ; Andreas Busch ; Michaella Georgiadou 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：25
页码：E4978-E4986
DOI：10.1073/pnas.1618539114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-negative species. To improve our limited understanding of the molecular mechanisms of filament assembly, we have used a synthetic biology approach to reconstitute, in a nonnative heterologous host, a minimal machinery capable of building Tfp. Here we show that eight synthetic genes are sufficient to promote filament assembly and that the corresponding proteins form a macromolecular complex at the cytoplasmic membrane, which we have purified and characterized biochemically. Our results contribute to a better mechanistic understanding of the assembly of remarkable dynamic filaments nearly ubiquitous in prokaryotes.
关键词：type IV pili ; type IV filamentous nanomachines ; filament assembly ; synthetic biology