文章基本信息

标题：Molecular codes for cell type specification in Brn3 retinal ganglion cells
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作者：Szilard Sajgo ; Miruna Georgiana Ghinia ; Matthew Brooks 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：20
页码：E3974-E3983
DOI：10.1073/pnas.1618551114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Visual information is conveyed from the eye to the brain by distinct types of retinal ganglion cells (RGCs). It is largely unknown how RGCs acquire their defining morphological and physiological features and connect to upstream and downstream synaptic partners. The three Brn3/Pou4f transcription factors (TFs) participate in a combinatorial code for RGC type specification, but their exact molecular roles are still unclear. We use deep sequencing to define ( i ) transcriptomes of Brn3a- and/or Brn3b-positive RGCs, ( ii ) Brn3a- and/or Brn3b-dependent RGC transcripts, and ( iii ) transcriptomes of retinorecipient areas of the brain at developmental stages relevant for axon guidance, dendrite formation, and synaptogenesis. We reveal a combinatorial code of TFs, cell surface molecules, and determinants of neuronal morphology that is differentially expressed in specific RGC populations and selectively regulated by Brn3a and/or Brn3b. This comprehensive molecular code provides a basis for understanding neuronal cell type specification in RGCs.
关键词：retinal ganglion cells ; transcription factors ; neuronal cell types ; Pou4f1 ; Pou4f2