文章基本信息

标题：In Vitro Production of Cholera Toxin by Vibrio cholerae El Tor Strains from Human Beings and the Environment
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作者：Kazumi YAMAGUCHI ; Yoshikazu NISHIKAWA ; Teruhiko KISHI 等
期刊名称：生活衛生
印刷版ISSN：0582-4176
电子版ISSN：1883-6631
出版年度：1988
卷号：32
期号：2
页码：71-74
DOI：10.11468/seikatsueisei1957.32.71
出版社：Osaka Urban Living and Health Association
摘要：The present study was undertaken to evaluate culture media for cholera toxin production by Vibrio cholerae El Tor and compare enterotoxigenicity among isolates from humans and the environment. A total of 20 strains of V. cholerae were cultured in syncase medium, CAYE medium, yeast extract peptone water and AKI medium. The culture supernatant was assayed for cholera toxin by reversed passive latex agglutination. The AKI medium was found best for the in vitro production of cholera toxin. The organisms were cultured in AKI medium at 37C for 4hr in a stationary test tube and then for 16hr in tube continuously shaken. Of 11 isolates from patients and the vicinity in which they lived, 10 produced cholera toxin, while 9 isolates from river water failed to do so.