文章基本信息

标题：ガスクロマトグラフィー/セレクテッドイオンモニタリングによるアコニチン系アルカロイドの分析
本地全文：下载
作者：水柿道直 ; 大山良治 ; 木村勝彦等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1988
卷号：34
期号：4
页码：359-365
DOI：10.1248/jhs1956.34.359
出版社：The Pharmaceutical Society of Japan
摘要：A gas chromatography/selected ion monitoring (GC/SIM) method for the simultaneous determination of aconitum alkaloids such as aconitine, mesaconitine and hypaconitine has been developed. Aconitum alkaloids were converted to their TMS ether derivatives by treatment with N, O-bis (trimethylsilyl) trifluoroacetamide in pyridine at room temperature. The gas chromatograms of reaction products showed in each case a well-resolved doublet consisting of a major (>70%) and a minor components, when analyzed using a 5%-phenylmethylsilicone cross-linked fused silica capillary column. These alkaloid derivatives gave simplified mass spectra with a base peak at m/z 698 for aconitine, m/z 684 for mesaconitine and m/z 596 for hypaconitine. When GC/SIM of aconitine was carried out under monitoring of the ion at m/z 698, detection limit was 10 picograms with signal-to-noise ratio of greater than 10. Linearity was obtained over a range from at least 10 to 1000pg. Similar results were obtained with another alkaloids. The use of this method for the measurement of aconitum alkaloids in biological fluids is described.
关键词：aconitine;mesaconitine;hypaconitine;TMS ether derivative;MS;GC/selected ion monitoring;microanalysis