文章基本信息

标题：前処理法に重層カラムクロマトグラフィーを用いた乳液, クリーム類中のグリチルリチン酸の定量
本地全文：下载
作者：三上栄一 ; 山田貞二 ; 早川順子等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1988
卷号：34
期号：5
页码：466-469
DOI：10.1248/jhs1956.34.466
出版社：The Pharmaceutical Society of Japan
摘要：A method was described for quantitative analysis of glycyrrhizin in cosmetic lotions and creams. Glycyrrhizin was separated from the cosmetic base and other ingredients by bi-layer (upper ; celite, lower ; silica gel) column chromatography with methanol containing 0.5% acetic acid. The eluate was washed with n-hexane. The methanol layer was concentrated in vacuo and determined by high-performance liquid chromatography (HPLC) on Develosil ODS using a mobile phase of acetonitrile-1% acetic acid (35 : 65). Methyl salicylate was employed as an internal standard. Recoveries of glycyrrhizin obtained from model cosmetics were more than 96% and coefficients of variation were less than 2.2%. This method with clean-up procedure on bi-layer column chromatography was much suitable for the determination of glycyrrhizin in cosmetic lotions and creams.
关键词：HPLC;glycyrrhizin;cosmetic;glycyrrhizin determination;bi-layer column chromatography