文章基本信息

标题：高性能薄層クロマトグラフィーによるパーマネント・ウェーブ用剤中のジチオジグリコール酸の定量
本地全文：下载
作者：臺隆男 ; 中村烈 ; 久保早苗等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1987
卷号：33
期号：4
页码：271-275
DOI：10.1248/jhs1956.33.271
出版社：The Pharmaceutical Society of Japan
摘要：A simple and rapid method by high performance thin layer chromatography (HPTLC) was studied for the determination of dithiodiglycolic acid (DTDGA) in hair-waving solutions. Sample and standard solutions were spotted on a silica gel plate. Immediately the plate was developed with diisopropylether-formic acid-water (90 : 7 : 3 v/v) for the separation of DTDGA and thioglycolic acid (TGA). The plate was sprayed with detecting solution : dissolve CuSO₄·5H₂O in a little distilled water, made up with methanol. DTDGA and TGA was visualized by heating for 30 min at 100°C. Densitometric determination was carried out with TLC scanner. DTDGA in the range of 0.5-20.0 μg was determined by the proposed method with spotting 1 μl of samples. And the HPTLC procedure in this paper was applicable to the quantitative analysis of DTDGA without an influence of other ingredients in hair-waving solutions.
关键词：high performance thin layer chromatography;dithiodiglycolic acid;densitometry;hair-waving solution;thioglycolic acid;HPLC