文章基本信息

标题：高速液体クロマトグラフィーによるポリリン酸塩の分析法 : 清涼飲料水への適用
本地全文：下载
作者：松永明信 ; 山本敦 ; 水上英一等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1988
卷号：34
期号：1
页码：70-74
DOI：10.1248/jhs1956.34.70
出版社：The Pharmaceutical Society of Japan
摘要：A high-performance liquid chromatographic method using post column reaction system was established for the determination of polyphosphates (PP). Linear polyphosphates with the range of polymerization from 2 to 9 (P2-P9) could be separated on an anion exchange column, TSK GEL DEAE-5PW (Toyo Soda), with 0.01 M HNO₃ containing NaNO₃ as a mobile phase at flow rate of 1.0 ml/min, with a linear gradient in NaNO₃ concentration at one hour intervals. PP were detected as the decrease of absorbance at 500 nm of ferric-sulfosalicylate complex by means of an on-line post column reaction at room temperature, with 0.5 mM FeCl₃ containing 2.5 mM sulfosalicylic acid as reaction reagent at flow rate of 0.5 ml/min. The proposed method was applied to the analysis of 30 different commercial products of soft drinks. The pretreatment only necessary after mixing the sample with an equal volume of 0.01 M HNO₃ was filtration through 0.45 μm pore size filter. P2-P6 (total 0.231 g/kg) and P2-P4 (0.070 g/kg) were found from 2 kinds of the samples, respectively. The determination limit was 0.02 m mol/kg for all of the PP.
关键词：HPLC;post column reaction method;determination;linear polyphosphate;pyrophosphate;tripolyphosphate;tetrapolyphosphate;soft drink