文章基本信息

标题：高速液体クロマトグラフィーによる河川水中の蛍光増白剤の定量
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作者：辻清美 ; 内藤昭治 ; 中澤裕之等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1988
卷号：34
期号：6
页码：501-507
DOI：10.1248/jhs1956.34.501
出版社：The Pharmaceutical Society of Japan
摘要：A simultaneous method for the determination of fluorescent Whitening agents (FWA), such as disodium 4, 4'-bis [(4-anilino-6-morpholino-1, 3, 5-triazin-2-yl) amino] stilbene-2, 2'-disulfonate (FB 71, trans isomer), disodium 4, 4'-bis [(4-toluidino-6-morpholino-1, 3, 5-triazin-2-yl) amino] stilbene-2, 2'-disulfonate (FB 225, trans isomer and cis isomer) and disodium 4, 4'-bis (2-sulfo-styryl) biphenyl (FB 351, trans isomer and cis isomer), in river waters with high performance liquid chromatography (HPLC) was developed. FWA in river waters were adsorbed on Sep-pak C₁₈ cartridge and eluted with methanol. The eluate was evaporated to dryness, and the residue was dissolved in acetonitrile-water (3 : 7). The solution was filtrated and analysed by HPLC under the following conditions : column, Inertsil ODS 5 (4.6 mm i.d.×150 mm) ; mobile phase, acetonitrile-0.01 M monobasic sodium phosphate (35 : 65) ; flow rate, 1.0 ml/min ; detections, ultraviolet detector (detection wavelength, 270 nm), fluorescent detector (excitation 345 nm and emission 430 nm). The average recoveries of FWA from river waters at 2-15.6 μg/l were 90-102%. This method was applicable to determine the concentration of 0.01μg/l for FB 351, 0.05μg/l for FB 71 (trans), 0.6μg/l for FB 71 (cis), 0.1μg/l for FB 225 (trans) and 1.1μg/l for FB 225 (cis), respectively, using 100 ml samples. On the basis of the present method applied to samples from 6 different rivers located at Kanagawa prefecture, FB 351 (trans), FB 71 (trans, cis) and FB 225 (trans, cis) were determined.
关键词：fluorescent whitening agent;river water;HPLC;stilbene type;detergent