文章基本信息

标题：Is K-ras Point Mutation an Early Event in Lung Tumorigenesis of Mice?
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作者：TOMIO ICHIKAWA ; YOSHIHISA YANO ; MIKAKO UCHIDA 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1996
卷号：42
期号：2
页码：178-181
DOI：10.1248/jhs1956.42.178
出版社：The Pharmaceutical Society of Japan
摘要：To investigate if K-ras point mutations could occur at an early stage of urethane-induced lung tumorigenesis in mice, we applied a sensitive mutation specific PCR assay technique (MSPA) to DNA from lung at the early stage of this condition. At 7 or 14 d after urethane injection (750 mg/kg, i. p.), pulmonary DNA was isolated and the existence of activated K-ras gene with 61st codon A→T mutation was checked according to the MSPA. This technique can detect mutant alleles in the presence of a very large excess of normal ras alleles ; however, the K-ras point mutation could not be detected by the present MSPA. These results suggest that the K-ras point mutations did not occur at the stage of lung tumorigenesis in mice or that the number of mutant alleles was below the detectable range of the technique.
关键词：K-ras point mutation;lung tumorigenesis;mutant specific PCR assay