文章基本信息

标题：尿試料の変異原性に及ぼす保存条件の影響
本地全文：下载
作者：遠藤治 ; 小谷野道子 ; 森康明等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1998
卷号：44
期号：5
页码：378-385
DOI：10.1248/jhs1956.44.378
出版社：The Pharmaceutical Society of Japan
摘要：Effects of storage conditions on the mutagenicity of human (2 smokers and 3 nonsmokers) urine were examined. Aliquots of urine from each male volunteer were stored at -80, 3, 25 and 40°C for various time points (max. 4 or 8 weeks). All extracts were obtained by the use of the Sep-pak tC₁₈/methanol method. At the end of maximum time points, all extracts were assayed by the pre-incubation method with Salmonella typhimurium YG1024. Some statistical changes of mutagenicity were observed under the storage conditions with 25 and/or 40°C for 1 or 2 weeks by the analysis of variance techniques. However, no statistical changes were observed under -80 and 3°C at least up to 4 weeks (significance level : 0.01). It was suggested that human urine samples for the mutagenicity assay may be practically stored at 3°C at least for 4 weeks.
关键词：human urine;Sep-Pak tC18;Salmonella typhimurium YG1024;stability;mutagenicity