文章基本信息

标题：生体試料中の8種有機リン殺虫剤の簡易定量法 : 中毒実験利用を目的として
本地全文：下载
作者：白坂正良 ; 紺野信弘
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1990
卷号：36
期号：4
页码：338-343
DOI：10.1248/jhs1956.36.338
出版社：The Pharmaceutical Society of Japan
摘要：A simple determination for eight kinds of organophosphorus pesticides (OPs) from animal tissues was newly developed. OP was extracted from the sample with acetonitrile, added 2% NaCl solution, and reextracted with n-hexane. The organic layer was loaded to a Sep-Pak silica cartridge and OP retained in the cartridge was eluted with ethyl acetate. The eluate was dried up under a stream of nitrogen. The residue was dissolved in n-hexane and the OP was determined by use of gas chromatograph (GC) equipped with a flame photometric detector (FPD). When nine OPs were separately added to four tissues (muscle, liver, adipose tissue and blood) of hen at the level of 5μg per 0.5 g of tissue weight, recoveries of fenitrothion, malathion, fenthion, parathion, cyanofenphos, EPN and leptophos ranged from 79.8% to 96.9%. In the case of cyanophos, recoveries from muscle, liver and adipose tissue were from 83.1% to 91.9%, but recovery from blood was 60.1%. Recoveries of dichlorvos from muscle, liver, adipose tissue and blood were below 18.8%. These results demonstrate that this simplified method is applicable to the determination of the above eight OPs except dichlorvos from animal tissues.
关键词：organophosphorus pesticide;Sep-Pak silica;FPD-GC;animal tissue