文章基本信息

标题：胆汁酸18種及びコレステロールとその代謝物等4種のキャピラリーGC及びGC/MSにおける挙動
本地全文：下载
作者：中村優美子 ; 長谷川ゆかり ; 外海泰秀等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1990
卷号：36
期号：5
页码：437-448
DOI：10.1248/jhs1956.36.437
出版社：The Pharmaceutical Society of Japan
摘要：Hexafluoroisopropyl ester-trifluoroacetate (HFIP-TFA) derivatives or methyl estertrifluoroacetate (Me-TFA) derivatives of 18 bile acids and non-derivatized or trifluoroacetate (TFA) derivatives of 4 neutral steroids containing cholesterol and its metabolites were analyzed by capillary GC. Non-polar or slightly polar columns were effective for the analysis of HFIP-TFA derivatives of bile acids. Non-polar columns were useful for the analysis of Me-TFA derivatives of bile acids. TFA derivatives of 4 neutral steroids were completely separated and analyzed by non-polar columns. Furthermore, HFIP-TFA derivatives of 18 bile acids and TFA derivatives of 4 neutral steroids were analyzed by capillary GC/MS with electron impact (EI) or chemical ionization (CI). Molecular ions ([M]⁺ or [MH]⁺) did not occasionally appear, but the specific fragment ions ([M-CF₃COOH]⁺ or [MH-CF₃COOH]⁺ and so on) were observed. Intensities of the specific fragment ions were tabulated.
关键词：bile acid;neutral steroid;capillary GC;capillary GC/MS;electron impact ionization;chemical ionization;hexafluoroisopropyl ester-trifluoroacetate derivative;methyl ester-trifluoroacetate derivative;trifluoroacetate derivative