文章基本信息

标题：化学発光検出高速液体クロマトグラフィを用いるヒト尿中覚せい剤分析法
本地全文：下载
作者：高山成明 ; 早川和一 ; 小林博等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1991
卷号：37
期号：1
页码：14-21
DOI：10.1248/jhs1956.37.14
出版社：The Pharmaceutical Society of Japan
摘要：High-performance liquid chromatographic method has been developed for the determination of trace levels of methamphetamine, amphetamine and piperidine in the human urine. Three compounds extracted into diethyl ether from alkaline urine were derivatized with dansyl chloride, and separated on a reversed-phase column and detected chemilumigenicaly by using bis (2, 4, 6-trichlorophenyl) oxalate and hydrogen peroxide as post column reagents. Methamphetamine as low as 2×10^-10M level in the urine was determined by the proposed method. The sensitivity of the proposed method was higher than that of gas-chromatography with flame ionization detection (FID-GC). The analytical results of methamphetamine in the urine samples of human who took methamphetamine by i.v. injection were in good agreement with those by FID-GC.
关键词：HPLC;dansyl chloride;chemiluminescence detection;methamphetamine;amphetamine;piperidine;human urine