文章基本信息

标题：Production of Aflatoxins and Aflatoxicols by Aspergillus flavus and Aspergillus parasiticus and Metabolism of Aflatoxin B1 by Aflatoxin-non-producing Aspergillus flavus
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作者：MITSUO NAKAZATO ; SATOSHI MOROZUMI ; KAZUO SAITO 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1991
卷号：37
期号：2
页码：107-116
DOI：10.1248/jhs1956.37.107
出版社：The Pharmaceutical Society of Japan
摘要：The production of aflatoxin (AF) groups (B₁, B₂, G₁, G₂, M₁, M₂, P₁ and Q₁) and aflatoxicol (AFL) groups (A and B) by AF-producing Aspergillus flavus and A. parasiticus isolated from various foods, and the metabolism of AF B₁ to AF M₁, AF P₁, AF Q₁, AFL-A and AFL-B by AF-non-producing A. flavus also isolated from various foods, were studied. A simple and rapid method for the analysis of the 10 kinds of toxins was developed. Toxins produced in culture were extracted with chloroform, separated into AF and AFL fractions by a silica gel cartridge column, then assayed by high performance liquid chromatography (HPLC). AF M₁, AF M₂, AFL-A and AFL-B were detected in addition to AF B₁ and AF B₂ in nearly all of the cultures inoculated with AF-producing A. flavus. The same groups were detected in addition to AF B₁, AF B₂, AF G₁ and AF G₂ in the cultures inoculated with A. parasiticus. The time courses of AF M₁ and AF M₂ production were almost the same as those of AF B₁, AF B₂, AF G₁ and AF G₂. AFLs tended to appear after AFs had accumulated in the cultures. AF P₁ and AF Q₁ were not detected at all. AF B₁ added to the cultures was metabolized by all strains of non-AF-producing A. flavus. AFL-A and AFL-B were the common metabolites in all of the cultures. However, no strains which converted AF B₁ to AF M₁, AF P₁ and AF Q₁ were observed.
关键词：mycotoxin;aflatoxin;aflatoxicol;Aspergillus flavus;Aspergillus parasiticus;HPLC;fluorescence detector;silica gel cartridge column;MS