文章基本信息

标题：活性汚泥に対する化学物質の影響と毒性評価法(第2報)酸素電極による酸素吸収速度の迅速定量とその応用について
本地全文：下载
作者：三原祐一 ; 古沢奈緒美 ; 秋葉雅子等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1991
卷号：37
期号：3
页码：179-184
DOI：10.1248/jhs1956.37.179
出版社：The Pharmaceutical Society of Japan
摘要：This paper deals with the investigation of simple and rapid method to determine the toxicity value (IC₅₀) of chemicals to activated sludge by using oxygen electrode. In this method, activated sludge was added to various concentrations of test chemicals and 50% inhibition of the oxygen uptake rate (OUR) was measured within 10 min. The IC₅₀ values calculated to be 1.5 mg/l, 1.5 mg/l, 4.0 mg/1, 7.2 mg/l, 14 mg/1, 14mg/l, 20 mg/l, 75 mg/l, 108 mg/l and 2500 mg/l for mercury (II) chloride, copper (II) chloride, mercury (II) acetate, copper (II) acetate, 2, 3, 5-trichlorophenol, pentachlorophenol, p-nitrophenol, 3, 4-dimethylphenol, cyclohexylamine and aniline, respectively, agreed almost well with those reported in the literatures. In addition, the OUR of activated sludge inhibited specifically by mercury (II) chloride was easily recovered by the addition of thiol compounds such as 2-mercaptoethanol, thiosulfate and reductive glutathione, respectively. Reactivation by thiol compounds suggested that mercury (II) form reversible mercaptides with sulfhydryl groups of enzymes concerned in OUR. From these results, the OUR method would be applicable to a rapid determination for biochemical field or biodegradation test in the Law Concerning the Examination and Regulation of Manufacture, etc. Chemical Substances.
关键词：activated sludge;oxygen uptake rate;toxicity;IC50 value;chemical