文章基本信息

标题：デプレニールとメタンフェタミンの尿中代謝物の比較に関する研究(第3報)キラール試薬GITCを用いたHPLCによるマウス尿中代謝物の光学異性体分離分析
本地全文：下载
作者：木倉瑠理 ; 石上暁子 ; 中原雄二等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1992
卷号：38
期号：2
页码：136-141
DOI：10.1248/jhs1956.38.136
出版社：The Pharmaceutical Society of Japan
摘要：Enantiomeric compositions of methamphetamine (MA) and its major metabolite, amphetamine (AP), in the urine of mice administered with deprenyl (DPN) and MA were analysed by high-performance liquid chromatography using the tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) derivatization method for the discrimination between the use of l-DPN and MA enantiomers. l-DPN, d-MA, l-MA and dl-MA were intraperitoneally administered to mice at 1, 5 and 10 mg/kg. Urine samples were collected at 0-12, 12-24, 24-48 and 48-72 h after administration, Urine added with N-methylphenethylamine (internal standard) was applied to BOND ELUT CERTIFY (1 ml), washed with 1 ml of 0.1 M acetic acid and 6 ml of methanol, and eluted with 2 ml of conc. HCl-methanol (9 : 91). The eluent was evaporated, redissolved in 1 ml of water, basified with 1 N NaOH and extracted with chloroform. The amphetamines in chloroform layer was derivatized with GITC at room temperature for 30 min. The GITC-derivatives were analysed by HPLC with a UV detector (254 nm). As a result, administration of l-DPN could be clearly discriminated from that of d-MA and dl-MA by comparisons of the enantiomeric compositions of MA excreted in the urine. Discrimination between administration of l-DPN and l-MA could be carried out by the apparent difference of ratio of AP/MA in the urine. The AP/MA ratio of the former was 10 times that of the latter.
关键词：urine analysis;deprenyl;methamphetamine;amphetamine;enantiomeric composition;HPLC;2, 3, 4, 6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC)