摘要:A method for the determination of swertiamarin (SA) in hair tonics containing Swertia japonica extract by use of high performance liquid chromatography (HPLC) was developed. A sample of hair tonic was evaporated twice with ethanol under reduced pressure and dissolved by adding water. The test solution was prepared by passing through a Sep-Pak C18 cartridge, washing with water and eluting with a mixture of acetonitrile-water (2 : 8). SA in the eluate was determined by HPLC on an octadecylsilylated silica gel column (TSKgel-ODS 80TM) by using a mobile phase of acetonitrile-water (12 : 88) and 235 nm for the detection. The present method was applicable to the determination of SA in commercial hair tonics without interference of other cosmetic ingredients.