文章基本信息

标题：マウス肝ミクロゾーム中のカンナビノール11位水酸化に関与する酵素系の諸性質について
本地全文：下载
作者：葛岡勝則 ; 渡辺和人 ; 松永民秀等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1992
卷号：38
期号：4
页码：349-354
DOI：10.1248/jhs1956.38.349
出版社：The Pharmaceutical Society of Japan
摘要：A mouse hepatic microsomal enzyme that catalyzes the oxidation of cannabinol (CBN) to 11-hydroxycannabinol (11-OH-CBN) was characterized. 11-OH-CBN formed was determined by HPLC. The reaction required NADPH as a cofactor and the optimal pH for the reaction was at 7.8. The Km and Vmax values for the reaction were 122 μM and 8.82 nmol/min/mg protein, respectively. The reaction was inhibited by inhibitors of cytochrome P450 such as SKF 525-A and by cannabidiol. The pretreatment of mice with phenobarbital significantly increased the enzyme activity but not with 3-methylcholanthrene, whereas that with cobaltous chloride significantly decreased the activity. These results indicate that cytochrome P450 plays a major role in the formation of 11-OH-CBN in the mouse hepatic microsomes.
关键词：cannabinol;cytochrome P450;11-hydroxycannabinol;enzymatic formation;hepatic microsomes;HPLC