文章基本信息

标题：o-Dinitrobenzene-核酸塩基付加物のSalmonella typhimurium株に対する変異原性並びにプリン塩基付加物の構造について
本地全文：下载
作者：平山晃久 ; 下村真司 ; 渡辺徹志等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1992
卷号：38
期号：4
页码：342-348
DOI：10.1248/jhs1956.38.342
出版社：The Pharmaceutical Society of Japan
摘要：o-Dinitrobenzene (DNBz) and each nucleic base were made to react with NaOH in dimethylsulfoxide at room temperature for 3-60 h. All of the synthesized compounds were the 2-nitrophenyl adducts of nucleic bases. The mutagenicity test of the synthesized compounds and of o-DNBz were then carried out against Salmonella typhimurium TA98, TA98NR and TA100 without a mammalian metabolic activation system. o-DNBz has no mutagenic potency against all the strains at a dose up to 100 μg. In the compounds with the adducts of nucleic bases, the mutagenic potencies of the adducts of adenine and guanine in TA98 were 50 revertants/100 μg and 255 revertants/10 μg, respectively and the most potent mutagen in TA100 was an adduct of guanine (382 revertants/100μg). Finally, the structures of the adducts of adenine and guanine could be elucidated as N¹-(2-nitrophenyl)-adenine and N¹-(2-nitrophenyl)-guanine, respectively, by the acid and alkaline degradation and by the isopentyl nitrite treatment of these adducts.
关键词：o-dinitrobenzene;adenine;guanine;cytosine;thymine;nucleic base adducts;mutagenicity test;Salmonella typhimurium strains;N1-(2-nitrophenyl)-adenine and guanine