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标题：Tetrachloroethylene Degradation by Culture Supernatant of the Anaerobic Bacterium, Strain T
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作者：KUMIKO YAGUCHI ; TOSHINARI SUZUKI ; ICHIRO HIRATA 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1994
卷号：40
期号：3
页码：252-258
DOI：10.1248/jhs1956.40.252
出版社：The Pharmaceutical Society of Japan
摘要：Degradation of tetrachloroethylene (PCE) by a gram negative, anaerobic bacterium strain T isolated from sludge in drainage from a laundry was examined. The activity of degradation was obtained in the culture supernatant of strain T, but not in the cell-extract by sonication. The reaction was optimal between pH 7.0 and 8.0 and at a temperature of 37°C. The activity was unstable in oxygen, heat labile and inhibited not only by trypsin and chymotrypsin but also by thiol inhibitors such as N-ethylmaleimide and p-hydroxymercuribenzoic acid. The substance degrading PCE in the culture supernatant of a gram negative anaerobic bacterium strain T might be a protein with the thiol group as an active site.
关键词：tetrachloroethylene;biodegradation;anaerobic bacterium;culture supernatant