文章基本信息

标题：Evidence for the Presence of Thiamin Diphosphate Kinase in Human Erythrocytes
本地全文：下载
作者：TADATOSHI YAMAGUCHI ; KINUKO UCHIMURA ; NORIKO MISHIRO 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1996
卷号：42
期号：6
页码：524-528
DOI：10.1248/jhs1956.42.524
出版社：The Pharmaceutical Society of Japan
摘要：A thiamin diphosphate (TDP) kinase (EC 2.7.4.15), which catalyzes the synthesis of thiamin triphosphate (TTP) from TDP, was found in erythrocytes after the freezing and thawing of human blood. The enzyme was biochemically distinct from the kinase previously reported. The enzyme activity was accelerated by Fe²⁺ and Fe³⁺ and inhibited by trichloroacetic acid, SH-blocking reagents and EDTA. It was suggested that the enzyme would have a sulfhydryl group at the active site, and requires certain cofactors for its activity.
关键词：thiamin diphosphate;thiamin triphosphate;thiamin diphosphate kinase;human erythrocyte