文章基本信息

标题：サンドイッチ酵素抗体法によるカラギーナンの測定について
本地全文：下载
作者：荒川正一 ; 西尾治 ; 宇野圭一等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1989
卷号：35
期号：1
页码：68-72
DOI：10.1248/jhs1956.35.68
出版社：The Pharmaceutical Society of Japan
摘要：Whether sandwich enzymeimmunoassay (EIA) could be used for the determination of x-carrageenan (x-CA) or not was studied. Furthermore, EIA was compared with double diffusion method and quantitative precipitation reaction. Antisera were obtained from rabbit and guinea pig immunized with x-CA, methylated bovine serum albumin and complete Freund's adjuvant. In EIA, the anti-x-CA rabbit serum as a capture antibody on the solid phase and anti-x-CA guinea pig serum as a detector antibody were used. Further, peroxidase-conjugated anti-guinea pig IgG rabbit antibody and a combination of hydrogen peroxide and orthophenylenediamine were used for color development. In EIA, most suitable dilutions for the determination of x-CA were 20000-fold in both rabbit and guinea pig antisera. The range of determination were from 64 to 512 ng/ml. Cross reaction with some additives with similar uses to x-CA was not observed except that agar slightly showed the reaction. EIA was superior in the sensitivity, the range of determination and the amount of antiserum required to two other methods, double diffusion method and quantitative precipitation reaction. Thus, it was suggested that EIA may be a useful method for the determination of x-CA.
关键词：x-carrageenan;enzymeimmunoassay;double diffusion method;quantitative precipitation reaction