文章基本信息

标题：高速液体クロマトグラフィーによる歯磨中のクロルヘキシジンの定量
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作者：岸美智子 ; 土井佳代 ; 堀口佳哉等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1989
卷号：35
期号：1
页码：49-54
DOI：10.1248/jhs1956.35.49
出版社：The Pharmaceutical Society of Japan
摘要：Determination of chlorhexidine in tooth paste was examined by using high performance liquid chromatography (HPLC). Chlorhexidine was extracted with methanol, isopropanol and acetonitrile. The best recovery was obtained with methanol containing acetic acid and sodium dodecyl sulfonate (SDS). Two g of sample was extracted with methanol-water-acetic acid (80 : 19.5 : 0.5) containing SDS (1%) and made up to 50 ml, and 20μl of this solution was injected to HPLC. A chlorhexidine peak was separated from other component peaks in commercial tooth paste by using an ODS column, 1-dodecansulfonate (DSA) as an ion pairing agent and methanol-water-THF (830 : 160 : 10) containing 5% acetic acid as an elution solution. The amounts of chlorhexidine in each 100 g of commercial tooth paste were ranged from 5.1 mg to 19.6 mg by this method.
关键词：chlorhexidine;HPLC;tooth paste;SDS