文章基本信息

标题：キャピラリー電気泳動法による目薬の成分分析
本地全文：下载
作者：岩上正藏 ; 沢辺善之 ; 中川照眞等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1995
卷号：41
期号：1
页码：85-91
DOI：10.1248/jhs1956.41.85
出版社：The Pharmaceutical Society of Japan
摘要：Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) with sodium dodecyl sulfate (SDS) as an anionic carrier were applied to the analysis of nine principal components of eye lotion. Simultaneous separation of these components except cyanocobalamin (VB₁₂), which has the same migration time with that of electroosmotic flow, was achieved by CZE using a 72 cm×50 μm i.d. fused silica capillary tube filled with 20 mM borate buffer (pH 10) at 25 kV DC voltage with UV detection at 200 nm. Under these conditions, the peak of chondroitin sulfate (CS) was overlapped with that of aspartic acid in case the latter was contained in the sample as a co-existing component. However, when pH was decreased to 9, CS was separated from other components and also from aspartic acid, and gave a more shapely peak. VB₁₂ could be separated from other components by MEKC with 20 mM borate buffer (pH 10) containing 40 mM SDS. The applicability to the determination of nine principal components of eye lotion is demonstrated.
关键词：eye lotion;capillary zone electrophoresis;micellar electrokinetic chromatography;simultaneous determination;sodium dodecyl sulfate