文章基本信息

标题：高速液体クロマトグラフィーによるトルエン吸入者の尿中馬尿酸の定量
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作者：神津公 ; 赤沼一弘
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1981
卷号：27
期号：2
页码：116-118
DOI：10.1248/jhs1956.27.116
出版社：The Pharmaceutical Society of Japan
摘要：Hippuric acid in the human urine was determined by high-performance liquid chromatography with a UV detector by use of stainless column (4.0 mm×0.15 m) packed with Nucleosil ₅C₁₈. Mobile phase was H₂O-methanol (80 : 20), adjusted to pH 3.0 with H₃PO₄. The sample solution was extracted with ethyl acetate after adjusting to pH 1.0 with 6N HCl. Determination was carried out successfully by using vanillic acid as an internal standard and by maintaining the temperature at 40°. The recovery of hippuric acid from aqueous solution (0.5 mg/ml) was 96.0±1.5%. The contents of hippuric acid detected in samples from suspected persons of toluene inhalation were 4.83, 16.01, 2.48 and 6.41 mg/ml, respectively.
关键词：high-performance liquid chromatography;reserved phase liquid-liquid chromatography;hippuric acid;vanillic acid;toluene