文章基本信息

标题：高速液体クロマトグラフィーによる安息香酸ナトリウムカフェインの定量
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作者：神津公
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1982
卷号：28
期号：2
页码：111-113
DOI：10.1248/jhs1956.28.2_111
出版社：The Pharmaceutical Society of Japan
摘要：Caffeine and sodium benzoate were determined by high performance liquid chromatography with a UV detector by use of stainless column (4.0 mm×0.15 m) packed with Nucleosil ₅C₁₈. Mobile phase was M/30 KH₂PO₄+M/30 Na₂HPO₄·2H₂O-methanol (70 : 30). The sample was dissolved in 20% aqueous methanol. Determination was carried out successfully by using antipyrine as an internal standard and by maintaining the temperature at 40°C. The recovery of sodium benzoate and caffeine from synthetic mixtures were 97.2±1.0% and 99.8±1.1%. This method was applied to three kinds of pharmaceutical preparations. The contents of sodium benzoate and caffeine were 48.4-50.1% and 49.0-49.6%.
关键词：high performance liquid chromatography;reserved phase liquid-liquid chromatography;caffeine;sodium benzoate;antipyrine