文章基本信息

标题：低濃度における有機リン農薬の生体影響に関する研究(第2報)低濃度におけるフェニトロチオンのマウス肝ホモジネートによる解毒
本地全文：下载
作者：河村葉子 ; 武田明治 ; 内山充等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1982
卷号：28
期号：2
页码：65-70
DOI：10.1248/jhs1956.28.2_65
出版社：The Pharmaceutical Society of Japan
摘要：Fenitrothion (FT) at low concentration of around 10^-6M reacted with mouse liver homogenate and disappeared immediately similarly to fenitrooxon (FO). This occurred mostly in the soluble fraction of mouse liver and was dependent on glutathione (GSH). The main metabolite detected was desmethyl-FT in the case of the reaction with a small amount of mouse liver homogenate, while desmethyl-FO was predominant metabolite in the reaction with relatively large amount of homogenate. In both cases all desmethylated metabolites were obtained more than 50% of added FT. These facts reveal that at such low concentration, FT is detoxified mainly through desmethylation reaction catalyzed by GSH S-transferase (GTase). On the other hand the amount of 4-nitro-m-cresol was less than 10%, so the oxidative dearylation by mixed function oxidase (MFO) contributed little to the detoxication of FT at low concentration. The Km and Vmax values of both GTase and MFO are well consistent with this reaction mechanism.
关键词：low concentration;fenitrothion;detoxication;mouse;in vitro;desmethyl-fenitrothion;desmethyl-fenitrooxon;4-nitro-m-cresol;glutathione S-transferase;mixed function oxidase