文章基本信息

标题：脂質過酸化物測定に影響を及ぼす金属イオンのアンバーリスト-15カラム前処理による除去
本地全文：下载
作者：宮崎元一 ; 早川和一 ; 飯田寿恵等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1982
卷号：28
期号：5
页码：282-285
DOI：10.1248/jhs1956.28.5_282
出版社：The Pharmaceutical Society of Japan
摘要：A simple method by using a column packed with Amberlyst-15 resin was established to remove metal ions which affect on POV and TBA tests for lipid peroxidation. A methanol solution (2 ml) of lipid containing metal ions was applied to the column (0.5 cm×5 cm), and eluted with methanol (6 ml). Metal ions such as Cu²⁺, Fe³⁺ and Mn²⁺ were retained on the column, whereas linoleic acid, linolenic acid and methyl linolenate were completely recovered in the eluate (8 ml). By testing the peroxidized lipid with POV and TBA methods, the recoveries of the lipid mixture after the procedure were more than 85% (POV) and more than 99% (TBA). This pretreatment by using Amberlyst-15 column is available for the peroxidation tests of lipid.
关键词：Amberlyst-15;POV;TBA;metal ion;lipid peroxidation;hydroperoxide;malondialdehyde