文章基本信息

标题：メチルオレンジ法によるモルヒネ定量の変法
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作者：小栗一太 ; 吉村英敏 ; 塚元久雄等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1968
卷号：14
期号：4
页码：217-220
DOI：10.1248/jhs1956.14.217
出版社：The Pharmaceutical Society of Japan
摘要：A little modification of the methyl orange method for the estimation of morphine which was developed by Woods, et al., was investigated. p-Nitrobenzoyl morphine was used to make its methyl orange complex by Woods, et al. On the other hand, the present modification involves selective acetylation of morphine with acetic anhydride in the presence of sodium bicarbonate and the extraction of the product, 3-acetylmorphine with chloroform. The derivative was then estimated by complexing with methyl orange. This method could be applied to the estimation of morphine in biological fluid, urine or plasma. Estimation of morphine was not affected by normorphine which is one of the metabolites of morphine.